RNA
Part:BBa_K3081104:Design
Designed by: Le Zhang Group: iGEM19_Peking (2019-10-16)
scaffold of sgRNA in CRISPR/Cas system
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
It already contains a terminator for DNA polymerase. But when you use some specific expression systems like T7, an extra corresponding terminator is needed.
Source
E.coli
References
Previous publication: Qi L S , Larson M H , Gilbert L A , et al. Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression[J]. Cell, 2013, 152(5):1173-1183.v Larson, M. H. , Gilbert, L. A. , Wang, X. , Lim, W. A. , Weissman, J. S. , & Qi, L. S. . (2013). Crispr interference (crispri) for sequence-specific control of gene expression. Nature Protocols, 8(11), 2180-2196.